To develop specific method for the detection of Salmonella A and D groups. rfbS genes were amplified by PCR. Amplified product of rfbS genes was identified by DNA sequencing analysis. The obtained results were as follows.
First, PCR products of rfbS were detected from Salmonella A and D Group only, but not from Salmonella B, C, E Group and other 11 bacteria.
Second, PCR was very sensitive to detect up to 100 pg of purified template DNA.
Third, rfbS genes of S. enteritidis, S. dublin and S. papatyphi A amplified, cloned and sequenced. The rfbS sequences of S. dublin and S. paratyphi A were compared with that of S. enteritidis. The homology of nucleotide sequences was 100%, 98.2%
respectively.
According to these results, PCR amplification of rfbS was rapid and sensitive to detect Salmonella A and D groups.
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